FrequentlyAskedQuestions

1. What is your timeline for developing an antibody?
Development takes approximately 3 months from the day the Purchase Order is received.
2. What is the affinity of your antibodies?
All our antibodies can detect picogram levels of their target in Western Blot.
3. Do I have to pay to get an antibody developed?
No, our antibody development service is completely free. You will only pay the price for purchasing the antibody, £387 for 100ul volume.
4. Why is your antibody development service free of charge?
Part of our mission is to develop an antibody to every protein used in research. We'd like to start with the proteins of immediate interest to researchers, to collaborate with them early and to tackle the reproducibility problem together. Once an antibody is developed, its recombinant source allows a forever reproducible supply. This allows us to capitialise on our initial efforts over time.
5. What is the Mix and Match deal?
The Mix and Match deal allows you to split your 100ul antibody volume between different conjugates in 25ul parts. For example, you can choose to purchase 50µl HRP, 25µl FITC and 25µl TRITC conjugates for any given antibody for the same price of £387.
6. Do you also provide unconjugated antibodies?
Unfortunately not. All our antibodies come conjugated with their detection signal. Part of our vision is to remove the need for secondary antibodies to allow for better reproducible research, less cross-reactivity, and multiplexing ability in the same tube.
7. What do we need to do to request antibodies for development?
We prefer to schedule a small zoom meeting to understand more about the target, the customer's experiments and the applications the antibody will be used in. Once satisfied with the information, we will send a quotation based on what conjugates the customer is interested in, and development can start when we have received the Purchase Order.
8. What main information do you need from us when placing an antibody development request?
We need:
  • Name of the target or its accession number.
  • The species that the customer is interested in.
  • The intended application the antibody will be used in.
9. My protein target is not a popular mouse or human target, can you still accept a request?
Yes, we can develop an antibody to any target of any species, as long as it's a protein target.
10. Can you develop antibodies against post-translational modifications?
Unfortunately, not for the moment.
11. Do you develop antibodies against lipids, fats or carbohydrates?
Unfortunately not, currently we are only taking in requests for protein targets.
12. Is there a limit to the number of antibodies we can request to be developed?
There is no limit, A customer can ask us to develop several antibodies in one single request and give several requests in any given year.
13. What if an antibody I ordered didn't work?
We ship 100µl HRP + 100µl fluorescent conjugated antibody with every order (total volume 200µl). Our guarantee is the antibody to work very specific in Western Blot, and with a high chance of it working in microscopy and flow cytometry applications.

The refund process can be initiated if there is a problem with the Western Blot application. We would require that the customer to send us a blot image and information on the conditions used, to make sure that all our recommendations on the datasheet have been followed.

  • If the blot proved to be non-specific, the purchase can be refunded.
  • If the blot didn't have any signal, we would require the customer to perform a dot blot (2-5µl of 1µg/µl lysate dotted on a membrane) to prove the absence of signal due to antibody and not transfer method. Once satisfied, the purchase can be refunded.
14. If I request an antibody to be developed, can you withhold it from other customers until I publish my data with it?
Unfortunately, we cannot. After we finish developing an antibody, it enters our catalogue and can be purchased by anyone interested.
15. Who owns the Intellectual Property of the antibody after the development?
Tresars would own 100% of all the Intellectual Property.
16. What applications are you currently focused on?
Currently, we can only develop antibodies intended to be used in WB, IHC, ICC, IF or FC.